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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP724396PSGYeast1mgQuote
EP724396PSGE. coli1mgQuote
BP724396PSGBaculovirus200ugQuote
MP724396PSGMammalian Cell200ugQuote

Protein Information

SpeciesPropionibacterium acnes (strain KPA171202 / DSM 16379)
UniProt IDQ6A8L1
Gene NamehisA; Locus:PPA1156
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMTGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFG HGSNTKIISSVVEQLDINVEVSGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVG RYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAGCRRYVVTDVASDGMLTGPNY ELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVC RAPIPADSSPMPPTD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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