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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP665608PTPYeast1mgQuote
EP665608PTPE. coli1mgQuote
BP665608PTPBaculovirus200ugQuote
MP665608PTPMammalian Cell200ugQuote

Protein Information

SpeciesPseudoalteromonas haloplanktis (strain TAC 125)
UniProt IDQ3ICF4
Gene NamehisA; Locus:PSHAb0489
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDVLQNQIVRLYQGKYETVQFYPFELGARLKEYADSGAGKLHLVDLEGARDPSKK QWQHIQAATKALNVPYQVGGGIRCEQDVSDWLKAGANQVVIGSMAVEKREQVKAWIEQFG AEHFVIALDVNKTATGWAPATHGWLTESEFGLLELVDFYANLGVIDFLCTDISKDGTMTG PSFALYEDLIKHNSTIKVQASGGVSSLDDIKKLKELGIGGIILGKSLLDGAFSVEEALAV TR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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