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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP658798Yeast1mgQuote
EP658798E. coli1mgQuote
BP658798Baculovirus200ugQuote
MP658798Mammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain CC9605)
UniProt IDQ3AIG3
Gene NamehisA; Locus:Syncc9605_1876
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMEIIPAIDLLDGACVRLHQGDYDQVTRFSEDPVAQALSWQSQGATRLHLVDLDGAKRGEP INDAAVRAITAALDIPVQLGGGVRSLERAEELISCGLDRVILGTVAIEQPELVQELAQRH PGRIVVGIDANDGRVATRGWIEQSDVLATDLAKQFSAAGIAAIITTDIATDGTLAGPNLE ALRTMAQCSAVPVIASGGIGCMADLLSLLPLEPLGVTGVIVGRALYDGRVDLAEAIAALG EARLQDVTAVAADIA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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