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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP655280Yeast1mgQuote
EP655280E. coli1mgQuote
BP655280Baculovirus200ugQuote
MP655280Mammalian Cell200ugQuote

Protein Information

SpeciesThiomicrospira crunogena (strain XCL-2)
UniProt IDQ31E65
Gene NamehisA; Locus:Tcr_1968
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGECVRLRQGLMDDATVFSGDIVAMAERWVNQGARRLHMVDLNGAFEGKP VNGDAVYQVREAFPDLPIQIGGGIRDLETIEAYLKAGVSYCIIGTKAVHNPEFVAEACKA FPGHIMVGLDAKEGMVAINGWAEVTDHNVIDLGKQFENDGVEAIIYTDIGRDGMMQGVNI QATQALAKALNIPIIASGGITNLDDIRALATIEKDGVSGAITGRAIYEGSLNFKEGQALS DELSKA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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