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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP651065Yeast1mgQuote
EP651065E. coli1mgQuote
BP651065Baculovirus200ugQuote
MP651065Mammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
UniProt IDQ2SUA7
Gene NamehisA; Locus:BTH_I2988
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-251
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGQCVRLKQGDMDQATIFSEDPAAMARKWVDLGARRLHLVDLNGAFAGKP KNLEAIEAILGEVGDEIPVQLGGGIRSLETIEKYLDAGLSYVIIGTAAVKDPGFLRDACS AFAGNIIVGLDAKDGKVATDGWSKLTGHEVIDLAQKFEDYGVESIVYTDIGRDGMLQGIN IEATVKLAQAVGIPVIASGGLSNIVDIEKLCEVEDEGIEGVICGRAIYSGDLDFAAAQKR ADELNGELDDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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