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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP672996Yeast1mgQuote
EP672996E. coli1mgQuote
BP672996Baculovirus200ugQuote
MP672996Mammalian Cell200ugQuote

Protein Information

SpeciesColwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus)
UniProt IDQ47XB4
Gene NamehisA; Locus:CPS_3894
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-261
Expression Tag6xHis
Purity>90%
AA SequenceMMIPAIDLIGGEVVRLYQGDYAQKTNYQYTVQDRQQAYAESGATVMHFVDLDGAKDSTKR QLKTLKTVVNHPSMIIQVGGGVRCEDDVKQLLALGADRVVIGSLAIKQPELVTQWVKTYG CEKIVLALDIKIDAQGNKTLPTHGWIEDSGVNLEDLLAQYQDAGIKHVLCTDISKDGTLT GTNVDLYSEVCAKYPDIDWQASGGIGSLADIKALIPTGVSGVILGRSLLEGKFTLEEAIA CWPKTSANNAESNTSSNTGDK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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