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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP674090Yeast1mgQuote
EP674090E. coli1mgQuote
BP674090Baculovirus200ugQuote
MP674090Mammalian Cell200ugQuote

Protein Information

SpeciesThermobifida fusca (strain YX)
UniProt IDQ47QS4
Gene NamehisA; Locus:Tfu_1155
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMAPILELLPAVDVAGGQAVQLVQGKDGTGGRYGDPLNAALTWQNAGAEWIHLVDLDAAFG RGHNRELLASIVGKLDIKVELSGGIRDDESLNAALATGCHRVNIGTAALENPDWCAKVIA EHGDRVAIGLDVRGTTLAARGWTRDGGDLYETLERLEAAGCARYIVTDVNKDGTLKGPNL DLLRDVCSRTDKPVVASGGISSLDDLRALAALVPEGVEGAIMGTALYEGAFTLEEALATV REVAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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