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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP671698DZRYeast1mgQuote
EP671698DZRE. coli1mgQuote
BP671698DZRBaculovirus200ugQuote
MP671698DZRMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha)
UniProt IDQ46WL7
Gene NamehisA; Locus:Reut_A3106
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-248
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGQCVRLKQGDMDQATVFSEDPAAMARHWVNQGARRLHLVDLNGAFVGKP RNEAAIKAIIAEVGDEIPVQLGGGIRDLNTIERWLDDGLSYVIIGTAAVKNPGFLKDACS AFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKYEDYGVEAIIYTDIGRDGMLQGIN IDATVKLAQSMSIPVIASGGLSNLADIDNLCAVENEGVEGVICGRAIYSGDLNFTDAQAR ADKLSNGE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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