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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP677297Yeast1mgQuote
EP677297E. coli1mgQuote
BP677297Baculovirus200ugQuote
MP677297Mammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain NATL2A)
UniProt IDQ46LD2
Gene NamehisA; Locus:PMN2A_0204
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-256
Expression Tag6xHis
Purity>90%
AA SequenceMEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAEIWESKGAKRLHLVDLDGAKTGEP INDLTIKEIKKSITIPIQLGGGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEY PKRIAVGIDAKEGMVATRGWLKQSEISSLDLAKQLNDLDLAAIISTDIATDGTLKGPNVQ ALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK NLLLQDAFNEKDKFLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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