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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP652536Yeast1mgQuote
EP652536E. coli1mgQuote
BP652536Baculovirus200ugQuote
MP652536Mammalian Cell200ugQuote

Protein Information

SpeciesSalinibacter ruber (strain DSM 13855 / M31)
UniProt IDQ2S296
Gene NamehisA; Locus:SRU_1564
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-258
Expression Tag6xHis
Purity>90%
AA SequenceMPLVIPAIDIRDGRCVRLHQGDYDNETVYFEDPVKMAKLWRVQNAQTLHVVDLDAARGEG EHNRDVIGKMCDALDIPIQLGGGIRSMDQIEAALDRGVYRVILGTAAVRNPDFVERAVEQ FSARRVVVSIDARDGEVRVQGWTEGSGLDAVAFAKDMEQRGVRRLVYTDISRDGTMDGPN IQAYRTLGRQLAHAKVTASGGVGEHDDLLDIQTLQPYGVDSVIVGTALYENRFPCQQFWA WQDKDAVDLDTFSTASLR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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