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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP652450MUGYeast1mgQuote
EP652450MUGE. coli1mgQuote
BP652450MUGBaculovirus200ugQuote
MP652450MUGMammalian Cell200ugQuote

Protein Information

SpeciesMoorella thermoacetica (strain ATCC 39073)
UniProt IDQ2RGW1
Gene NamehisA; Locus:Moth_2032
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMLVMPAIDLREGRCVRLYQGRIEAETVYSTDPVAVARGWVERGARWLHVVDLDGAFAGRP RNLEVIAAIIRAAGVPVQVGGGIRSLESLAGVLAAGASRVVLGTVAITNPEVVATAVERY GERVVVGIDSLDGQVAIEGWEATVARGAVEFARQMASLGVTRAVFTDIGRDGTLQGPNIA ATREMARSGGLKIIASGGIASLDDLRKLKELAAEGVEGAILGRSLYNGNFTLEEALAVAA DGD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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