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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP676420Yeast1mgQuote
EP676420E. coli1mgQuote
BP676420Baculovirus200ugQuote
MP676420Mammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)
UniProt IDQ48C80
Gene NamehisA; Locus:PSPPH_4923
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDLKDGACVRLRQGRMEDSTVFSDDPVAMAAKWVDGGCRRLHLVDLNGAFEGQP VNGDVVTAIARRYPNLPIQIGGGIRSLETIEHYVKAGVSYVIIGTKAVKEPEFVAEACRA FPGKVIVGLDAKDGFVATDGWAEVSSVQVIDLAKRFEADGVSAIVYTDIAKDGMMQGCNI PFTAALAAATRIPVIASGGIHNLGDIQALLNAKAPGIIGAITGRAIYEGTLDVAEAQALC DREQR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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