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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP667779Yeast1mgQuote
EP667779E. coli1mgQuote
BP667779Baculovirus200ugQuote
MP667779Mammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194) )
UniProt IDQ39K86
Gene NamehisA; Locus:Bcep18194_A3528
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-251
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGQCVRLKQGDMDQATIFSEDPAAMARKWVDLGARRLHLVDLNGAFAGKP KNLEAIEAILDEVGDEIPVQLGGGIRSLETIEKYLDAGLSYVIIGTAAVKDPGFLQDACT AFAGSIIVGLDAKDGKVATDGWSKLTGHEVIDLAQKFEDYGVESIVYTDIGRDGMLQGIN IEATVKLAQAVGIPVIASGGLSNLVDIENLCEVEEQGVEGVICGRAIYSGDLDFAAAQKR ADELNGELDNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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