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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP655256Yeast1mgQuote
EP655256E. coli1mgQuote
BP655256Baculovirus200ugQuote
MP655256Mammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9312)
UniProt IDQ31B81
Gene NamehisA; Locus:PMT9312_0804
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-255
Expression Tag6xHis
Purity>90%
AA SequenceMELIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKYWESEGAKYIHIVDLDAAKTGSP TNDKSIKDIAKTVNIPIQIGGGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKF PGRIIVGIDAKDGKVSTRGWLEQSNILATDLVREFSSFKIASFIVTDINTDGTLEGTNEE FIKRILEITDIPVIASGGVGSISDLLSLVKLENSGLFGVIVGKALYENKFKISEAINVLS SERLTDFDLNNNYYA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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