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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP653761Yeast1mgQuote
EP653761E. coli1mgQuote
BP653761Baculovirus200ugQuote
MP653761Mammalian Cell200ugQuote

Protein Information

SpeciesSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251) )
UniProt IDQ30PY1
Gene NamehisA; Locus:Suden_1673
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-236
Expression Tag6xHis
Purity>90%
AA SequenceMTLYPAIDLKDGKAVRLTKGLMDSAKIYSDEPWMLVKKFEEMGAKWVHLVDLNGAFAGEP KNLEQIIKIRQNCKVKLELGGGIRDEDTIKKMLEIGIDRVILGSIAVKNPTFVKEMAAKY PIAVGIDAIDGFVAVEGWGEVSAMRATDLAKEFANAGVEAIICTDVSRDGTLSGVNVEFT LDIANACKIPTIASGGVKDESDIEALVSAKGIDGVIIGKAYYEGTLDLPKMFKKYS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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