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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP626308Yeast1mgQuote
EP626308E. coli1mgQuote
BP626308Baculovirus200ugQuote
MP626308Mammalian Cell200ugQuote

Protein Information

SpeciesDeinococcus geothermalis (strain DSM 11300)
UniProt IDQ1IWD2
Gene NamehisA; Locus:Dgeo_2158
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMTPVSSRLQPLIIPCVDIQSGRAVRLYEGDPDRETVYFESPLEAARHWAALGAGLVHLVD LDAATGRGENRAVITQITAELGVPVEVGGGIRDRAAAEALLDAGVGRVVIGTAAVKNPAL VRELIAAHGPSRVVVSLDARGLDVAIHGWAEGSGVSVAELAPLLAEAGLETLIFTDVTRD GTLRGLDRDLMRQVRQLWSNTLIVGGGVATLDDVRLLAEEGIQGAIVGRAIYEGTLVYPV TGL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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