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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP631453Yeast1mgQuote
EP631453E. coli1mgQuote
BP631453Baculovirus200ugQuote
MP631453Mammalian Cell200ugQuote

Protein Information

SpeciesSaccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
UniProt IDQ21NH6
Gene NamehisA; Locus:Sde_0489
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMIVIPAIDLKDGACVRLRQGLMEDSTVFSDDPAAMARKWVEQGAKRLHLVDLNGAFAGEP VNGEVVKAIAKAYPNLPIQIGGGIRSLETIKHYLDAGVTYVIIGTKAVKEPEFVKQACDA FPGHIIVGLDAKDGYVATDGWAEVSTVKATDLAKQFAADGVSEIVYTDIARDGMMQGVNI EATVEMARAGNIPIIASGGITNMDDIKGLMAVAHEGITGAITGRAIYEGSLDLAEAQAYC DAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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