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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP626115Yeast1mgQuote
EP626115E. coli1mgQuote
BP626115Baculovirus200ugQuote
MP626115Mammalian Cell200ugQuote

Protein Information

SpeciesSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
UniProt IDQ1GNC2
Gene NamehisA; Locus:Sala_3147
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-244
Expression Tag6xHis
Purity>90%
AA SequenceMAALTIFPAIDLKAGQVVRLAEGDMDRATVYGDDPAAQARRFADAGATHLHVVDLDGAFA GASVNGAAVESIIAAFPGQVQVGGGIRDRAGVDRWLALGVARVIIGTAALKDPEFVKSAA RDLPGRIVVGVDARDGMVATEGWADVSDVRVEDLARRFEDAGVAALLFTDVGRDGLLKGC NVAATVALAGVVDIPVIASGGVAGIEDIHALRPHVADGIEGVITGRALYDGRLDLAEAIA AGAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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