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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP623687Yeast1mgQuote
EP623687E. coli1mgQuote
BP623687Baculovirus200ugQuote
MP623687Mammalian Cell200ugQuote

Protein Information

SpeciesPolaromonas sp. (strain JS666 / ATCC BAA-500)
UniProt IDQ12FC8
Gene NamehisA; Locus:Bpro_0808
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGHCVRLKQGDMDQSTIFSEDPAAMARSWVDKGARRLHLVDLNGAFAGKP KNEAAIKKILAEVGSEIDVQLGGGIRDLDTIERYLDAGLRYVIIGTAAVKNPGFLQDACT AFGGHIIVGLDAKDGKVATDGWSKLTGHEVIDLGKKFQDYGVESIIYTDIGRDGMLSGIN IEATVRLAQALTIPVIASGGLSNMADIEALCDVEEEGVEGVICGRSIYSGDLDFAAAQAR ADELNG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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