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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP633038Yeast1mgQuote
EP633038E. coli1mgQuote
BP633038Baculovirus200ugQuote
MP633038Mammalian Cell200ugQuote

Protein Information

SpeciesJannaschia sp. (strain CCS1)
UniProt IDQ28NK3
Gene NamehisA; Locus:Jann_2792
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMILYPAIDLKDGNAVRLLRGEMSDATVFNTDPAAQARAFQDAGCDWLHLVDLNGAFAGEP VNGAAVEAILAATSVPTQLGGGIRDMATIEGWLTRGIARVILGTVAVEDPDLVRQAARAF PGQVAVGIDARKGRVATKGWAEETDVMVTDLARAFEDAGVAAIIYTDIDRDGAMGGPNVS ATADLARATSIPVIASGGVSSLDDLHALKTTGVISGAISGRALYDGALDLSEALHALA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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