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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP635451Yeast1mgQuote
EP635451E. coli1mgQuote
BP635451Baculovirus200ugQuote
MP635451Mammalian Cell200ugQuote

Protein Information

SpeciesRhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118)
UniProt IDQ21U92
Gene NamehisA; Locus:Rfer_2950
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGHCVRLKQGDMDQSTTFSEEPFVMARNWVDKGARRLHLVDLNGAFAGHP KNELAIRKILKDVGSEVDVQLGGGIRDLDTIERYLDAGLRYVIIGTAAVKNPGFLQDACT AFGGHIIVGLDARDGKIATDGWSKLTRHDVVDLAKKFEDYGVESIIYTDISRDGMLSGIN IEATVRLAQALTIPVIASGGLSGMADIEALCAVENEGIEGVICGRAIYSGDLDFEAAQER ANELNG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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