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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP627057Yeast1mgQuote
EP627057E. coli1mgQuote
BP627057Baculovirus200ugQuote
MP627057Mammalian Cell200ugQuote

Protein Information

SpeciesMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
UniProt IDQ1H4R3
Gene NamehisA; Locus:Mfla_0253
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-247
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDLKDGHCVRLKQGLMEDATVFSEDPGAMARHWVDQGGKRLHLVDLNGAFAGKP VNEGAIKAIVKAVGNDIPIQLGGGIRDLDTIERYLDDGISYVIVGTAAVKSPGFLHEACY AFPGQIMVGLDAKDGKVAVDGWSKLTGHDVIDLAKKFEDYGVEAVVYTDIGRDGMLTGVN IEATVALAQALTIPVIASGGVTNLDDVRRLAEVESEGIIGAITGRAIYEGTLNFTEAQAL ADELSQI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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