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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP643350Yeast1mgQuote
EP643350E. coli1mgQuote
BP643350Baculovirus200ugQuote
MP643350Mammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain JA-3-3Ab) (Cyanobacteria bacterium Yellowstone A-Prime)
UniProt IDQ2JRV8
Gene NamehisA; Locus:CYA_2513
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-261
Expression Tag6xHis
Purity>90%
AA SequenceMPHASAFLEVIPAIDLLQGRAVRLYQGDYAQAEQVAADPVQQAEIWATQGAPRLHVVDLD GAKSGDPVNLPIIADIVRKLAIPVQVGGGIRSLERARQLLDLGVERVIVGTVAVEDPALL EAMTQALPGRIWVGIDARQGQVATRGWLSTTPLAATELVQRVQAQGAAGIIYTDIGRDGT LAGPNLEQLRQILAVSRLPVIASGGIGSLTDLLALLSLPGLAGAILGKALYSGAISLPEA LRAVGPGRWQDLPPETGSRWA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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