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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP645482SSGYeast1mgQuote
EP645482SSGE. coli1mgQuote
BP645482SSGBaculovirus200ugQuote
MP645482SSGMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain JA-2-3B'a (2-13) ) (Cyanobacteria bacterium Yellowstone B-Prime)
UniProt IDQ2JK82
Gene NamehisA; Locus:CYB_1964
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-261
Expression Tag6xHis
Purity>90%
AA SequenceMPYTSAFLEVIPAIDLLQGRAVRLYQGDYAQAEQVADDPIQQAEAWAAQGAPRLHVVDLD GAKSGDPVNLPIIERIVRSLAIPVQVGGGIRSLERARQLLDLGVDRVIVGTLAVEDPDTL EAMTQAFPGRVWVGIDARQGQVATRGWLSTTPLRAPELVQRVQAQGAAGIIYTDISRDGT LAGPNLEQLRQILAVSQVPVIASGGIGSLTDLLALLSLPRLTGAILGKALYSGAISLQEA LRAVGPGRWQDLPPETGSLWA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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