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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP641440Yeast1mgQuote
EP641440E. coli1mgQuote
BP641440Baculovirus200ugQuote
MP641440Mammalian Cell200ugQuote

Protein Information

SpeciesFrankia sp. (strain CcI3)
UniProt IDQ2J8L2
Gene NamehisA; Locus:Francci3_3023
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-252
Expression Tag6xHis
Purity>90%
AA SequenceMTLTLLPAVDVADGRAVRLVQGEAGSETSYGDPREAALTWQRDGAEWIHLVDLDAAFGRG SNRELIAEVVRAVDVAVELSGGIRDDASLDAALATGAARVNIGTAALEDPDWVRRAIDRV GDRIAVGLDVRGTTLSARGWTRDGGELFDVLARLDADGCARYVVTDVRRDGTLTGPNVEL LRSVTAATSRPVVASGGVATLDDLTAIAVVPGVEGAIIGKALYAGAFTLPEALAVAGNIG NIGNGCAGAVGR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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