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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP602459Yeast1mgQuote
EP602459E. coli1mgQuote
BP602459Baculovirus200ugQuote
MP602459Mammalian Cell200ugQuote

Protein Information

SpeciesSyntrophomonas wolfei subsp. wolfei (strain Goettingen)
UniProt IDQ0AW40
Gene NamehisA; Locus:Swol_1766
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-244
Expression Tag6xHis
Purity>90%
AA SequenceMIIYPAIDLKDGQCVRLVQGRVENKTVYSDAPANVARSFQEQGASFLHIVDLDGAFQGSP QNLKAIEAIAAAIDISFQVGGGLRQLSDVEKLLALGASRVIIGTRAVSHPDFIANLLDKF GPERIVLGLDAKEGMVAVEGWVSTSSVSAIDFGLQMKALGIQTAIYTDVSRDGLLQGPNL AAIKEMASSTGLDIIASGGVSSLDNIRALKELENDGVSGAIIGKALYDGKIGLVDALREA ESKF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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