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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP602721Yeast1mgQuote
EP602721E. coli1mgQuote
BP602721Baculovirus200ugQuote
MP602721Mammalian Cell200ugQuote

Protein Information

SpeciesMaricaulis maris (strain MCS10)
UniProt IDQ0AP83
Gene NamehisA; Locus:Mmar10_1612
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-244
Expression Tag6xHis
Purity>90%
AA SequenceMILYPAIDLLDGRVVRLHKGAFDAVTDYGDDPMRVAEQFGEAGAVWVHIVDLSGARDGAR RQGALIRSLCETGLRVQTGGGVRSAEDIEDLLAAGVERVIIGSLAVTDTNRVAGWLQNYG GDRITLALDVRQIGGQYRPALKGWTDMADTTLDDVISAYEGTGLAHALVTDIGRDGDLSG PNIALYKRLATDYPNIDWQASGGVSSLDDLRAARAAGAAGAITGKALYEGRFTVGEAIAC LRDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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