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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP645597RKKYeast1mgQuote
EP645597RKKE. coli1mgQuote
BP645597RKKBaculovirus200ugQuote
MP645597RKKMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium etli (strain CFN 42 / ATCC 51251)
UniProt IDQ2KE60
Gene NamehisA; Locus:RHE_CH00043
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-248
Expression Tag6xHis
Purity>90%
AA SequenceMILFPAIDLKGGQCVRLKLGDMQQATVYNTDPAAQAKSFEDQGFEWLHVVDLDGAFAGHS ANGDAVEAILKATKNPVQLGGGIRTLDHIEAWLSRGLRRVILGTVAVRNPELVIEACRKF PGRVAVGIDAKGGKVAVEGWAEASELGIIELAKKFEGAGVAAIIYTDIDRDGILTGINWS STLELADAVSIPVIASGGLASLNDIKRMLQPDARKLEGAISGRALYDGRIDPKEALALIN ANRAKETA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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