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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP651514Yeast1mgQuote
EP651514E. coli1mgQuote
BP651514Baculovirus200ugQuote
MP651514Mammalian Cell200ugQuote

Protein Information

SpeciesSodalis glossinidius (strain morsitans)
UniProt IDQ2NTX5
Gene NamehisA; Locus:SG1125
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDLIDGAVVRLHQGDYQTQCHYGADPLPRLQDYLRQGAEVLHLVDLTGARDPQTR QIPLLRRLLAGVAPALVQVGGGIRSAADVEVLLEAGATRVVVGSTAVLQPQEVQRWFERF GPDALVLALDVRIDDDGQRRVAISGWQEDSGATLEQVITQYRPLGLKHVLCTDISRDGTL TGSNVSLYRSLCDAWSDIAFQSSGGIGSLADIAQLRHSGVQGVIVGRSLLENKFTVAEAL ACWQNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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