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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP649108Yeast1mgQuote
EP649108E. coli1mgQuote
BP649108Baculovirus200ugQuote
MP649108Mammalian Cell200ugQuote

Protein Information

SpeciesMethanosphaera stadtmanae (strain DSM 3091)
UniProt IDQ2NHB3
Gene NamehisA; Locus:Msp_0389
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-237
Expression Tag6xHis
Purity>90%
AA SequenceMIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGR NLSIVKEIIEKTKSPIQLGGGIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYG CERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGAGSILFTNVDVEGLLNGINLE PIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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