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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP301877SSQYeast1mgQuote
EP301877SSQE. coli1mgQuote
BP301877SSQBaculovirus200ugQuote
MP301877SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP74561
Gene NamehisA; Locus:slr0652
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-256
Expression Tag6xHis
Purity>90%
AA SequenceMKILPAIDLLGGKCVRLYQGDYDQSQVYHEDPVEVARQWQAQGASRLHLVDLDGAKEGQP VNLTAIANIVEALTIPVQVGGGLRDRNRVKQLLDLGVGRVILGTIAVENPDLVGELCAEF PGQIVVGIDARNGKVATRGWLETSTVEAGELAQRMEKLGAAAIIYTDIHRDGTMQGPNLD ALRQLASQLTIPVIASGGVSQVEDLLNLLSLESLGVNGVIIGKALYTGDIQLAEAIRAVG NGRWQDVPPLDFPRLG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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