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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516578BRJYeast1mgQuote
EP516578BRJE. coli1mgQuote
BP516578BRJBaculovirus200ugQuote
MP516578BRJMammalian Cell200ugQuote

Protein Information

SpeciesBacillus subtilis (strain 168)
UniProt IDO35006
Gene NamehisA; Locus:BSU34880
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMSAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKE GKRVNDRHVIEIAQKLNLKVEIGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKML KQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELANEGAEVFIFTDIATDGMLSGP NVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALE RVKRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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