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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP475509TMYYeast1mgQuote
EP475509TMYE. coli1mgQuote
BP475509TMYBaculovirus200ugQuote
MP475509TMYMammalian Cell200ugQuote

Protein Information

SpeciesThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
UniProt IDB5YIB4
Gene NamehisA; Locus:THEYE_A1955
Protein Name
Region Expressed1-244
Expression Tag6xHis
Purity>90%
AA SequenceMQIIPAIDLKDGKCVRLRQGKFSEVTVYYDNPEDAALRWQNEGAEVLHVVDLDGAKEGKI GNLPSIKKIREAFRGNIEVGGGIRRIEDIELLLSVGIDRVIVGTIAVQSPDFVKEVCKRF PKKIIVGIDAKDGLVAVKGWVEVTEIRAIELALKMQDYGIWGIIYTDISRDGMLTGPNIE ATKALVESVKVPVIASGGVSSIEDIRKLAEIPQLWGVITGKAIYSGAIDLKEALRIIKGD GVKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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