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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP543594DZYYeast1mgQuote
EP543594DZYE. coli1mgQuote
BP543594DZYBaculovirus200ugQuote
MP543594DZYMammalian Cell200ugQuote

Protein Information

SpeciesCyanothece sp. (strain ATCC 51142)
UniProt IDB1WRP7
Gene NamehisA; Locus:cce_4140
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMDVIPAIDLLEGRCVRLYQGNYQQSEVYNENPVEVARQWADEGATRLHLVDLDGAKQGHP VNLDTIEAIVRAISIPVQVGGGLRDRQSIAQLIDLGVDRTIVGTVAVENPSLVQELCQAF PSKIAVGIDARNGKVATRGWLETSEVLATELAQQMAELGVSAIIYTDIHRDGTLQGPNRE ALRELANHIKIPVIASGGVSSLSDVLGLLALEPIGVTGVIIGKALYTGDVSLAEAVHAVG PGRWQDIPPDLGSSAFG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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