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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP484861DZZYeast1mgQuote
EP484861DZZE. coli1mgQuote
BP484861DZZBaculovirus200ugQuote
MP484861DZZMammalian Cell200ugQuote

Protein Information

SpeciesCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155) )
UniProt IDB7KEI6
Gene NamehisA; Locus:PCC7424_4950
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMEVIPAIDLLDGRCVRLYQGDYQQSQVFSENPVEVARQWVEQGANRLHLVDLDGAKAGKP VNLSAIEAIVRAVSIPVQVGGGLRDRSSVAQLLNLGVNRVILGTIAVENPQLVQQLSQEF PGQIVVGIDAREGKVATRGWLETSEVQATELAQNMAHLGVAAIIYTDIHRDGTLKGPNIP ALRELATAVNVPVIASGGVSSLTDLLSLLALEPSGVTGVIVGRALYTGEVDLSEAIQAVG QGRWQDIPPDLGSSAFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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