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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP482240EPWYeast1mgQuote
EP482240EPWE. coli1mgQuote
BP482240EPWBaculovirus200ugQuote
MP482240EPWMammalian Cell200ugQuote

Protein Information

SpeciesCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1) )
UniProt IDB7K3A9
Gene NamehisA; Locus:PCC8801_0331
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMDVIPAIDLLDGRCVRLYQGDYQQSQVYNDNPVEVARQWADQGATYLHLVDLDGAKQGKP VNLASIEAIIRGISIPVQVGGGLRDRASIVQLFNLGVDRAIVGTVAVENPTLVKELCAEF PRKIAVGIDARNGKVATRGWLETSEVIATELAQEMAQLGAAAIIYTDIHRDGTLSGPNRE ALRELASNIEIPVIASGGISSLTDLLGLLSLEPLGVTGVIVGKALYTGDVDLSEALRAIG PGRWQDVPPDIDSSLFG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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