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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP470089AWHYeast1mgQuote
EP470089AWHE. coli1mgQuote
BP470089AWHBaculovirus200ugQuote
MP470089AWHMammalian Cell200ugQuote

Protein Information

SpeciesAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270) )
UniProt IDB7JA18
Gene NamehisA; Locus:AFE_3045
Protein Name
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKGGNCVRLRQGRMEDDTVFSDDPVATAQRWVEAGAKRLHIVDLDGAVQGEP VNAHAIAAICAGFPDLEIQVGGGIRSEEQIETYIQAGVRYVIIGTQAVKAPGFVADATVS FPGHIMVGIDARDGKVATEGWSKLSRHDPIDLAQRFAADGIEAIIYTDISRDGMLSGPNI SATVALAQAVPVPVIASGGIANLEQVLALKAHESDGITGAITGRAIYEGTLDFSQARAQA EA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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