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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488215EOBYeast1mgQuote
EP488215EOBE. coli1mgQuote
BP488215EOBBaculovirus200ugQuote
MP488215EOBMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O127:H6 (strain E2348/69 / EPEC)
UniProt IDB7UT61
Gene NamehisA; Locus:E2348C_2165
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAEVLHLVDLTGAKDPAKR QIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVGSTAVKSPEMVKGWFERFG ADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRDGTLA GSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTGVRGVIVGRALLEGKFTVKEAIA CWQNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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