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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP480355BYTYeast1mgQuote
EP480355BYTE. coli1mgQuote
BP480355BYTBaculovirus200ugQuote
MP480355BYTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain K)
UniProt IDB4UDJ9
Gene NamehisA; Locus:AnaeK_0751
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMLVIPAIDLIGGEVVRLEKGDFARKTVYARDPAEKAAELVRDGASLIHVVDLDGAKAGWP VNLDAVRAICAVPGAEVELGGGLRSLPDIEKVLELGVRYVVLGTAAVERLDLVRQACARF PGRVRSGIDARNGEVKIAGWLEGTGLGAAEVARRVKEAGVGLVEYTDVGRDGMFTGVDAE GAARLQAEAGVQVVASGGVAGLDDVRACRAAGLAGVIVGKALYEGRIALAEAVRAAAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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