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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP416216AUTYeast1mgQuote
EP416216AUTE. coli1mgQuote
BP416216AUTBaculovirus200ugQuote
MP416216AUTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain Fw109-5)
UniProt IDA7H8C3
Gene NamehisA; Locus:Anae109_0756
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMLVIPAIDLMGGEAVRLEKGDFATKTVYARHPAEKAEEFARAGATLLHVVDLDGAKAGWP VNLDAVRAICEVPGIEVELGGGLRSLPDIEKVLALGVRYVVLGTAAVERLGLVEQACQRF PGQVRAGIDARNGEVKIAGWLEGTGLAAVDVARKVKGAGVGLVEYTDVARDGMFTGVDAA GAARIQAEAGIPVVASGGVASLDDVTACRAAGLAGVIVGKALYERRIDLAAAIRAAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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