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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP433482HSKYeast1mgQuote
EP433482HSKE. coli1mgQuote
BP433482HSKBaculovirus200ugQuote
MP433482HSKMammalian Cell200ugQuote

Protein Information

SpeciesHerpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
UniProt IDA9B5I3
Gene NamehisA; Locus:Haur_0156
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-240
Expression Tag6xHis
Purity>90%
AA SequenceMQLIPAIDLRDGRCVRLVQGDFEQTTVYGDDPVAVAQQWEAAGAARIHIVDLDGAKAGRP TQTSTIAAITKAVSVPVQLGGGLRDAASIAAAFELGVSDVILGTVAVRNPELVAELVRQY GKAITIGIDARNGVVATEGWLASSRQRATELAEQMGQLGVARIIYTDISRDGTLSEPNYA QTAALVTPNGPAIIASGGIARVEHLARLAELGISGAIIGTALYTGHIDLASAIQTIEHGG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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