1- (5-phosphoribosyl) -->

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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog Number	Expression Host	Amount	In Stock
YP415144VEA	Yeast	1mg	Quote
EP415144VEA	E. coli	1mg	Quote
BP415144VEA	Baculovirus	200ug	Quote
MP415144VEA	Mammalian Cell	200ug	Quote

Protein Information

Species	Vibrio harveyi (strain ATCC BAA-1116 / BB120)
UniProt ID	A7MX07
Gene Name	hisA; Locus:VIBHAR_01835
Protein Name	1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed	1-245
Expression Tag	6xHis
Purity	>90%
AA Sequence	MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTAR QLDLIAKLLASTPANIQIGGGVRTEQDVIDLLEAGAQRVVVGSTAVKQPELVKGWMEKYG AEKIVLALDINIYQDGTRKVAISGWQEDSGVTIEALINDYLTVGLQHVLCTDISRDGTLE GSNVELYVDLCKQYPQVQFQSSGGIGSLADIEALKGSGVAGVIVGRALLDGKFTAEEAFA CWQSE
Storage Buffer	PBS pH 7.4, 50% glycerol
Storage	Store working aliquots at 4^oC for upto a week. Freeze at -20 to -80^oC for long term storage. Avoid repeated freeze/thaw.

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