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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP392223BPVYeast1mgQuote
EP392223BPVE. coli1mgQuote
BP392223BPVBaculovirus200ugQuote
MP392223BPVMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808) )
UniProt IDA4JAW8
Gene NamehisA; Locus:Bcep1808_0409
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-251
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKDGQCVRLKQGDMDQATIFSEDPAAMARKWVDLGARRLHLVDLNGAFAGKP KNLEAIEAILEEVGDEIPVQLGGGIRSLETIEKYLDAGLSYVIIGTAAVKNPGFLQDACT AFSGSIIVGLDAKDGKVATDGWSKLTGHEVIDLARKFEDYGVESIVYTDIGRDGMLQGIN IDATVKLAQAVGIPVIASGGLSNLTDIENLCEVEEHGVEGVICGRAIYSGDLDFAAAQKR ADELNGELDDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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