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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP390203GEHYeast1mgQuote
EP390203GEHE. coli1mgQuote
BP390203GEHBaculovirus200ugQuote
MP390203GEHMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus thermodenitrificans (strain NG80-2)
UniProt IDA4ISR3
Gene NamehisA; Locus:GTNG_3022
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMASFTIYPAIDMRGGKCVRLLQGDYNKETVYGDSPVAMAEQFAAQGAEWIHMVDLDGAKE GRRVNDRFVIEAANRLSVNVQVGGGIRTEEDVAYYLERGVARVILGSAAISNPTFVKKML QTYGRRIVIGIDARDGFVATEGWLETSNVKAEELGQMLAEAGAETFIFTDIATDGTLSGP NITAAVRLAEATGKEVIASGGVRSLDDLRALREYAEQGIGGAIVGKALYTNQFTLAEALK AVNER
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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