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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP368669MNGYeast1mgQuote
EP368669MNGE. coli1mgQuote
BP368669MNGBaculovirus200ugQuote
MP368669MNGMammalian Cell200ugQuote

Protein Information

SpeciesMagnetococcus sp. (strain MC-1)
UniProt IDA0LCF2
Gene NamehisA; Locus:Mmc1_3155
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-249
Expression Tag6xHis
Purity>90%
AA SequenceMRIIPAIDLKDGQCVRLFKGDMDQNTVYSDNPGETAKQWAEQGAERMHVVDLNGAFAGEP VNADAIAAIRKAITIPMQLGGGIRTLETLQKLFNLGVDFAILGSVAARDPELVFRACEQF PGRISVGIDARDGKVAVEGWAETTDLNAVDLAKKFEDAGVAEIIFTDIARDGTLTGPNVA ATRLMAESATIPVIASGGVSCLADIQALLENSGPYPNGNRISGVITGKAIYDGRLDLAAA IALSRRWEN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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