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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400204LLJYeast1mgQuote
EP400204LLJE. coli1mgQuote
BP400204LLJBaculovirus200ugQuote
MP400204LLJMammalian Cell200ugQuote

Protein Information

SpeciesLegionella pneumophila (strain Corby)
UniProt IDA5IB92
Gene NamehisA; Locus:LPC_0662
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMLVIPAIDLQSGRCVRLKQGRFDQVTQFSVFPIERALHFAKLGAKRLHVVDLDGARSGKM QQLELICSMQKTGIPIQAGGGIRSIEQALECSSAGISQLVIGSLAITNTDLTIQIIEKIK PENIVLALDVRVDTKVPLLAINGWQNNSTSSLWEVVSYYENYGIKNILCTDIACDGMMNG PNFDLYQQAVEYFPQIAWQASGGIRHMQDITTLGSLGISAVILGLMLYQDNVSFEELLC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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