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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400440GEIYeast1mgQuote
EP400440GEIE. coli1mgQuote
BP400440GEIBaculovirus200ugQuote
MP400440GEIMammalian Cell200ugQuote

Protein Information

SpeciesGeobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens)
UniProt IDA5G8T0
Gene NamehisA; Locus:Gura_4054
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIVIPAIDLKEGKCVRLEQGLMDKDTVFCDNPAEQAREWERQGGELLHIVDLDGAFAGKP ANKSAIEAIVKAVRIPTQLGGGIRDIETIKAYLDLGLSRVILGTAAQRNPKLVEEACKLF PGRIVVGIDAKNGLVAVQGWAEVTDVYAVDLARLFEGYGVTAVIYTDISRDGMMQGPNID ATRALAEAISIPVIASGGVSSLKDIENLMAVESAGVTGVITGKAIYTGAIKLAEAVALTK KCRQG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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