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Recombinant 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component

Recombinant 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP304392ENVYeast1mgQuote
EP304392ENVE. coli1mgQuote
BP304392ENVBaculovirus200ugQuote
MP304392ENVMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12)
UniProt IDP77650
Gene NamehcaD; aka: hcaA4, phdA, yfhY; Locus:b2542, JW2526
Protein Name3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component
Region Expressed1-400
Expression Tag6xHis
Purity>90%
AA SequenceMKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQ QVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA LGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELAASATQRRCKVTVIELAATVM GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL IGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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