1. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells.<br />To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 30, 60, minutes, respectively by adding the recombinant protein (100ng/ml corresponding to a Specific Activity of 100IU/mg) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60mins, respectively.<br />2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 10, 30, 60mins, respectively by adding the recombinant protein(500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60mins, respectively.
The protein solution contains 1x PBS.
Sterile Filtered clear solution.
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Progranulin although stable at 4oC for 3 weeks, should be stored at -20oC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
GRN, PGRN, granulin, Acrogranin, propithelin, PC cell derived growth Factor, GEP, GP88, PEPI, PCDGF.
