Growth Hormone Placental 20kDa Human Recombinant
Growth Hormone Placental 20kDa Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. Growth Hormone 20K placental is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs.
GH 20K placental is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile.
Formulation
pGH 20kDa was lyophilized from a concentrated (1mg/ml) solution with0.0045mM NaHCO3 previously adjusted pH 11.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein content
GH 20K Placental quantitation was carried out by two independent methods::
1. UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH 20K as a Reference Standard.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Placental Growth Hormone in 0.4% NaHCO3or water adjusted to pH 11, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Growth Hormone 20K Placental although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution and filter sterilization GH 20K pl can be stored at 4oC for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
GHL, GHV, GH-V, hGH-V, PGH.
